Conaghan J, Hardy K, Handyside A H, Winston R M, Leese H J
Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
J Assist Reprod Genet. 1993 Jan;10(1):21-30. doi: 10.1007/BF01204436.
Pyruvate uptake is higher in human embryos developing to the blastocyst stage than those arresting at cleavage stages. To investigate whether pyruvate uptake provides an improved criterion for selecting embryos for transfer, we have measured uptakes by individual embryos noninvasively over 24-hr periods between the first day (day 1) postinsemination and embryo transfer on day 2 to 3 and correlated the levels with implantation and pregnancy outcome.
The mean uptake was significantly lower for embryos that implanted than for those which failed to implant: 22.9 +/- 1.0 and 27.1 +/- 0.6 pmol/embryo/hr, respectively on day 2, and 22.4 +/- 1.5 and 26.9 +/- 0.8 pmol/embryo/hr, respectively, on day 3, but the wide range of uptakes by individual embryos was overlapping.
We conclude that pyruvate uptake as the sole criterion for embryo selection cannot predict which embryos will implant after transfer. Assessment of embryos using morphological and developmental criteria, therefore, remains the most consistent, though inefficient, indicator of pregnancy potential.
发育至囊胚阶段的人类胚胎比在卵裂阶段停滞发育的胚胎对丙酮酸的摄取更高。为了研究丙酮酸摄取是否能为选择移植胚胎提供更好的标准，我们在授精后第1天至胚胎移植日（第2至3天）的24小时内，对单个胚胎的丙酮酸摄取进行了非侵入性测量，并将摄取水平与着床和妊娠结局相关联。
着床胚胎的平均摄取量显著低于未着床胚胎：第2天分别为22.9±1.0和27.1±0.6皮摩尔/胚胎/小时，第3天分别为22.4±1.5和26.9±0.8皮摩尔/胚胎/小时，但单个胚胎的摄取量范围有重叠。
我们得出结论，以丙酮酸摄取作为胚胎选择的唯一标准无法预测哪些胚胎在移植后会着床。因此，使用形态学和发育标准评估胚胎仍然是预测妊娠潜力最一致但效率不高的指标。
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