改进DNA脉冲场琼脂糖凝胶电泳程序的进展。
Progress in developing improved programs for pulsed field agarose gel electrophoresis of DNA.
作者信息
Arshad M F, Dunn F J, Vega R, Valvano J W, Serwer P
机构信息
Department of Electrical and Computer Engineering, University of Texas, Austin.
出版信息
Electrophoresis. 1993 Apr;14(4):344-8. doi: 10.1002/elps.1150140158.
Details are described here for using a rotating gel to perform pulsed field agarose gel electrophoresis (PFGE) with programmable control of the following variables: magnitude of the electrical field, polarity of the electrical field, temperature of the gel and position of the rotating disk upon which the agarose gel rests. By use of this procedure for programmable control, modes of PFGE have been explored that have the following characteristics: (i) resolution by DNA length is completely lost for DNA shorter than a critical length that increases as the pulse times increase, and (ii) resolution by DNA length is enhanced for longer DNAs that are shorter than a second critical length. This window of resolution can be moved to the position of the 2-6 Mb chromosomes of Schizosaccharomyces pombe.
本文详细介绍了使用旋转凝胶进行脉冲场琼脂糖凝胶电泳（PFGE）的方法，可对以下变量进行可编程控制：电场强度、电场极性、凝胶温度以及琼脂糖凝胶所放置的旋转盘的位置。通过使用这种可编程控制程序，探索了具有以下特征的PFGE模式：（i）对于短于临界长度的DNA，按DNA长度的分辨率完全丧失，且随着脉冲时间增加，临界长度增大；（ii）对于短于第二个临界长度的较长DNA，按DNA长度的分辨率增强。这种分辨率窗口可移动到粟酒裂殖酵母2 - 6 Mb染色体的位置。
Zotero 插件