Yan K, Collier J R
Department of Biology, City University of New York, Brooklyn College 11210.
Dev Biol. 1993 Jun;157(2):303-7. doi: 10.1006/dbio.1993.1136.
We have immunostained Ilyanassa embryos with an antibody to the ras protein and shown that the ras gene (1) is not expressed from early cleavage through the gastrula stage, but (2) is expressed during organogenesis by both normal and lobeless embryos, (3) that the ras protein is first synthesized from the third day of development in the normal embryo and from the fifth day of development in the lobeless embryo, and (4) that the expression of the ras protein is localized in mesodermal lineages of both normal and lobeless embryos.
我们用针对ras蛋白的抗体对伊利亚纳萨胚胎进行了免疫染色，结果表明，ras基因：（1）从早期卵裂到原肠胚阶段均不表达；（2）在器官发生过程中，正常胚胎和无叶胚胎均有表达；（3）正常胚胎从发育的第三天开始首次合成ras蛋白，无叶胚胎从发育的第五天开始合成；（4）ras蛋白的表达定位于正常胚胎和无叶胚胎的中胚层谱系中。
