Fox J G, Blanco M, Murphy J C, Taylor N S, Lee A, Kabok Z, Pappo J
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge 02139.
Infect Immun. 1993 Jun;61(6):2309-15. doi: 10.1128/iai.61.6.2309-2315.1993.
Helicobacter felis inoculated per os into germfree mice and their conventional non-germfree counterparts caused a persistent chronic gastritis of approximately 1 year in duration. Mononuclear leukocytes were the predominant inflammatory cell throughout the study, although polymorphonuclear cell infiltrates were detected as well. Immunohistochemical analyses of gastric mucosa from H. felis-infected mice revealed the presence of mucosal B220+ cells coalescing into lymphoid follicles surrounded by aggregates of Thy-1.2+ T cells; CD4+, CD5+, and alpha beta T cells predominated in organized gastric mucosal and submucosal lymphoid tissue, and CD11b+ cells occurred frequently in the mucosa. Follicular B cells comprised immunoglobulin M+ (IgM+) and IgA+ cells. Numerous IgA-producing B cells were present in the gastric glands, the lamina propria, and gastric epithelium. Infected animals developed anti-H. felis serum IgM antibody responses up to 8 weeks postinfection and significant levels of IgG anti-H. felis antibody in serum, which remained elevated throughout the 50-week course of the study.
经口接种到无菌小鼠及其常规非无菌对照小鼠体内的猫幽门螺杆菌，引发了持续约1年的慢性胃炎。在整个研究过程中，单核白细胞是主要的炎症细胞，不过也检测到了多形核细胞浸润。对感染猫幽门螺杆菌小鼠的胃黏膜进行免疫组织化学分析发现，黏膜B220+细胞聚集成淋巴滤泡，周围有Thy-1.2+ T细胞聚集；在有组织的胃黏膜和黏膜下淋巴组织中，CD4+、CD5+和αβ T细胞占主导，CD11b+细胞在黏膜中频繁出现。滤泡性B细胞包括免疫球蛋白M+（IgM+）和IgA+细胞。胃腺、固有层和胃上皮中有大量产生IgA的B细胞。感染动物在感染后8周内产生了抗猫幽门螺杆菌血清IgM抗体反应，血清中抗猫幽门螺杆菌IgG抗体水平显著升高，在整个50周的研究过程中一直保持在较高水平。
