单细胞胚胎活检及Y连锁睾丸特异性基因分析后对小鼠进行精确的性别选择生育。
Precise sex selected births of mice following single cell embryo biopsy and Y-linked testis-specific gene analysis.
作者信息
Cui K H, Putland R A, Seamark R F, Matthews C D
机构信息
Department of Obstetrics and Gynaecology, Queen Elizabeth Hospital, Woodville, S.A., Australia.
出版信息
Hum Reprod. 1993 Apr;8(4):621-6. doi: 10.1093/oxfordjournals.humrep.a138107.
Abstract
We report on the birth of 39 mice following single cell embryo biopsy and precise sex determination following in-vitro fertilization. Polymerase chain reaction was used to amplify fragments of the mouse testis-specific gene sequence (pYMT2/B) on the Y chromosome and the ovary-specific gene (ZP3) sequence on chromosome 5 from the single biopsied cell. Embryo biopsy was not associated with any deleterious effects.
摘要
我们报告了经单细胞胚胎活检及体外受精后精确性别鉴定出生的39只小鼠。采用聚合酶链反应从单个活检细胞中扩增Y染色体上小鼠睾丸特异性基因序列（pYMT2/B）片段以及5号染色体上卵巢特异性基因（ZP3）序列。胚胎活检未产生任何有害影响。
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