用于肺炎克雷伯菌脂多糖分型和定量的酶联免疫吸附测定方法的开发：应用于O1血清型
Development of an enzyme-linked immunosorbent assay method for typing and quantitation of Klebsiella pneumoniae lipopolysaccharide: application to serotype O1.
作者信息
Albertí S, Hernández-Allés S, Gil J, Reina J, Martínez-Beltrán J, Camprubí S, Tomás J M, Benedí V J
机构信息
Universidad de las Islas Baleares, Palma de Mallorca, Spain.
出版信息
J Clin Microbiol. 1993 May;31(5):1379-81. doi: 10.1128/jcm.31.5.1379-1381.1993.
Abstract
We describe a method for the typing and quantitation of Klebsiella pneumoniae serotype O1 lipopolysaccharide (LPS) based on inhibition in an enzyme-linked immunosorbent assay of a reaction of known O1 LPS antigen and anti-O1 antibody by unknown LPS extracts. Serotype O1 was found in 32% of the 124 K. pneumoniae clinical isolates tested, showing that this serotype is frequent among the eight O serotypes which have been described previously.
摘要
我们描述了一种基于在酶联免疫吸附测定中，未知脂多糖提取物对已知O1脂多糖抗原与抗O1抗体反应的抑制作用，来对肺炎克雷伯菌O1血清型脂多糖（LPS）进行分型和定量的方法。在所检测的124株肺炎克雷伯菌临床分离株中，32%发现有O1血清型，这表明该血清型在先前已描述的8种O血清型中较为常见。
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