Crutcher K A, Scott S A, Liang S, Everson W V, Weingartner J
Department of Neurosurgery, University of Cincinnati Medical Center, Ohio 45267-0515.
J Neurosci. 1993 Jun;13(6):2540-50. doi: 10.1523/JNEUROSCI.13-06-02540.1993.
A two-site ELISA and a bioassay were used to detect NGF-like activity in human brain tissue. Both assays detected mouse NGF and recombinant human NGF with approximately equal sensitivity, whereas the antibodies showed little cross-reactivity with the recombinant human proteins NT-3 and brain-derived neurotrophic factor. NGF-like activity was detected in fresh human cortical samples obtained from epileptic patients, with the highest activity observed in the right hemisphere of men. NGF-like activity was subsequently measured in autopsy samples of frontal and occipital cortex from patients with Alzheimer's disease (AD) and from individuals with no history or pathological evidence of AD. Based on both the ELISA and the bioassay measurements, NGF-like activity was significantly elevated in both brain regions in AD. These results demonstrate the feasibility of detecting NGF-like activity in both fresh and postmortem human brain tissue and further suggest that AD is characterized by increased, rather than decreased, levels of cortical beta-NGF. The AD-related increase in NGF may be a consequence of degenerative changes in the basal forebrain cholinergic system.
采用双位点酶联免疫吸附测定法（ELISA）和生物测定法检测人脑组织中的类神经生长因子（NGF）活性。两种测定法对小鼠NGF和重组人NGF的检测灵敏度大致相同，而这些抗体与重组人蛋白NT-3和脑源性神经营养因子几乎没有交叉反应。在从癫痫患者获取的新鲜人皮质样本中检测到了类NGF活性，在男性的右半球中观察到的活性最高。随后在患有阿尔茨海默病（AD）的患者以及无AD病史或病理证据的个体的额叶和枕叶皮质尸检样本中测量了类NGF活性。基于ELISA和生物测定法的测量结果，AD患者的这两个脑区中的类NGF活性均显著升高。这些结果证明了在新鲜和死后的人脑组织中检测类NGF活性的可行性，并进一步表明AD的特征是皮质β-NGF水平升高而非降低。AD中NGF的相关增加可能是基底前脑胆碱能系统退行性变化的结果。
