Cooke P S, Kirby J D, Porcelli J
Department of Veterinary Biosciences, University of Illinois, Urbana 61801.
J Reprod Fertil. 1993 Mar;97(2):493-9. doi: 10.1530/jrf.0.0970493.
Treatment of male rat pups with the reversible goitrogen 6-propyl-2-thiouracil (PTU), administered by adding 0.1% PTU to the mother's drinking water from birth to day 25, increases their testis size and daily sperm production (DSP) at 160 days of age by up to 80% and 140%, respectively. The purpose of this study was to examine the effectiveness of various concentrations of PTU and determine the PTU dose that would maximize testis growth while minimizing side effects such as decreased maternal water consumption and decreased pup growth. Whether this effect was specific to PTU was determined by evaluating the effects of another commonly used goitrogen, methimazole (MMI), in increasing adult testis size and sperm production. Dams were given PTU (0.1-0.0004% w/v) or 0.025% MMI (w/v) in their water from birth to day 25 post partum, then given no further treatment. Thyroxine concentrations were measured in all groups of pups at 25, 35 and 45 days, and testis weight and DSP were determined at 90 days of age. At 25 days of age, thyroxine concentrations were maximally decreased by PTU treatments of 0.0015% or greater; less severe decreases were produced by 0.0004% PTU or 0.025% MMI. Thyroxine concentrations increased in all treated groups at day 35 compared with day 25, and returned to normal by day 45. At 90 days of age, testis weight was increased by about 40% in rats whose mothers had been treated with doses of 0.006% PTU or greater, whereas testis weights in groups given 0.0015 and 0.0004% PTU or 0.025% MMI were increased 31, 15 and 18%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
从出生到第25天，通过在母鼠饮水中添加0.1%的可逆性致甲状腺肿物质6-丙基-2-硫氧嘧啶（PTU）来处理雄性幼鼠，可使它们在160日龄时的睾丸大小和每日精子生成量（DSP）分别增加高达80%和140%。本研究的目的是检验不同浓度PTU的有效性，并确定能使睾丸生长最大化同时将诸如母鼠饮水量减少和幼鼠生长减缓等副作用降至最低的PTU剂量。通过评估另一种常用的致甲状腺肿物质甲巯咪唑（MMI）对成年睾丸大小和精子生成的影响，来确定这种效应是否特定于PTU。从出生到产后第25天，给母鼠的饮水中添加PTU（0.1 - 0.0004% w/v）或0.025% MMI（w/v），之后不再进行进一步处理。在25、35和45天时测量所有幼鼠组的甲状腺素浓度，并在90日龄时测定睾丸重量和DSP。在25日龄时，0.0015%及以上的PTU处理可使甲状腺素浓度最大程度降低；0.0004% PTU或0.025% MMI导致的降低程度较轻。与25天时相比，所有处理组在35天时甲状腺素浓度均升高，并在45天时恢复正常。在90日龄时，母亲接受0.006%及以上剂量PTU处理的大鼠睾丸重量增加约40%，而接受0.0015%和0.0004% PTU或0.025% MMI处理组的睾丸重量分别增加31%、15%和18%。（摘要截断于250字）
