Ishikawa I, Shikura N, Ishii H, Fukuda Y
Department of Internal Medicine, Kanazawa Medical University, Uchinada, Japan.
Nihon Jinzo Gakkai Shi. 1993 Mar;35(3):257-63.
We observed that administration of exogenous glycerol reduces beta-ATP level and increases glycerophosphate level using magnetic resonance spectroscopy (MRS) of the kidney. A new "glycerol-loading test" for use with MRS in rats was developed. Sprague-Dawley rats were anesthetized and the left kidney was exposed and placed on surface coil. 31P-MRS (109.25MHz) of the kidney was performed using 10 microseconds, 90' pulse width and 2.308 sec repetition time with an accumulation of 200 times. MRS was performed 24 hours after induction of acute renal failure in each model of acute renal failure. Glycerol-loading test was performed by the intravenous infusion of 10% glycerol through jugular vein over a 60 min period. MRS was recorded every 10 min during glycerol infusion and for 60 min after cessation of infusion. In normal rats, beta-ATP level in the kidney was decreased to 52.0% of the pre-loading value at 55 min glycerol infusion and recovered to 66.7% 55 min after glycerol infusion was stopped. In the cisplatinum model, the reduction of beta-ATP and the increase of glycerophosphate levels following glycerol-loading was similar to that in normal rats; however, the recovery of beta-ATP level after infusion was stopped was weaker. Rats treated with glycerol and HgCl2 showed rather severe acute renal failure, but the beta-ATP level at 55 min glycerol infusion was 87.3% and 92.4%, respectively, showing difference from that in normal rats. No uptake of glycerol was observed 3 hours after 45 min pedicle clamping.(ABSTRACT TRUNCATED AT 250 WORDS)
我们通过肾脏磁共振波谱（MRS）观察到，外源性甘油的给药会降低β-ATP水平并增加磷酸甘油水平。开发了一种用于大鼠MRS的新“甘油负荷试验”。将Sprague-Dawley大鼠麻醉，暴露左肾并置于表面线圈上。使用10微秒、90°脉冲宽度和2.308秒重复时间，累积200次，对肾脏进行31P-MRS（109.25MHz）检查。在每种急性肾衰竭模型中，急性肾衰竭诱导24小时后进行MRS检查。通过颈静脉在60分钟内静脉输注10%甘油进行甘油负荷试验。在甘油输注期间每10分钟记录一次MRS，并在输注停止后记录60分钟。在正常大鼠中，甘油输注55分钟时，肾脏中的β-ATP水平降至负荷前值的52.0%，甘油输注停止55分钟后恢复至66.7%。在顺铂模型中，甘油负荷后β-ATP的降低和磷酸甘油水平的升高与正常大鼠相似；然而，输注停止后β-ATP水平的恢复较弱。用甘油和HgCl2处理的大鼠表现出相当严重的急性肾衰竭，但甘油输注55分钟时的β-ATP水平分别为87.3%和92.4%，与正常大鼠不同。在45分钟肾蒂夹闭后3小时未观察到甘油摄取。（摘要截短至250字）
