Matsuda K, Taki T, Hamanaka S, Kasama T, Rokukawa C, Handa S, Yamamoto N
Department of Microbiology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Biochim Biophys Acta. 1993 Jun 12;1168(2):123-9.
Glycolipid compositions of cells infected by human retroviruses (human immunodeficiency virus, HIV and/or human T-cell lymphotropic virus type I, HTLV-I) have been studied. Eight cell lines, comprising two HTLV-I-infected T-cell lines (MT-2 and MT-4), two HTLV-I-negative T-cell lines (Jurkat and MF), a macrophage cell line (U937), and three HIV-infected counterpart cell lines (MT-4/HIV, Jurkat/HIV and U937/HIV) were used. The neutral glycolipids and gangliosides isolated from these cell lines were compared. Among them, the HTLV-I-infected T-cell lines, MT-2 and MT-4, showed similar patterns for both neutral glycolipids and gangliosides. Neutral glycolipids (GlcCer and LacCer) of MT-2 and MT-4 cells were markedly decreased, and a ganglioside, GM3, of theirs was decreased to only a trace amount compared to that in other cell lines. Gangliosides of MT-4 and MT-4/HIV were further separated on an Iatrobeads column, and were identified as GM2, GM1a and GD1a by methylation and liquid secondary ion mass spectrometric analyses. Since the patterns of neutral glycolipids and gangliosides of MT-2 and MT-4 are unique, as compared to those of HTLV-I-negative cells, it is suggested that these changes are related to HTLV-1 infection. No prominent differences in the ganglioside compositions between HIV-infected and non-infected cell lines could be observed. But it is noteworthy that the contents of asialo-GM2 in Jurkat/HIV and MT-4/HIV cells were increased as compared to those in the parental cell lines.
对感染人类逆转录病毒（人类免疫缺陷病毒，HIV和/或人类T细胞白血病病毒I型，HTLV-I）的细胞的糖脂组成进行了研究。使用了8种细胞系，包括2种感染HTLV-I的T细胞系（MT-2和MT-4）、2种HTLV-I阴性T细胞系（Jurkat和MF）、1种巨噬细胞系（U937）以及3种对应的感染HIV的细胞系（MT-4/HIV、Jurkat/HIV和U937/HIV）。对从这些细胞系中分离出的中性糖脂和神经节苷脂进行了比较。其中，感染HTLV-I的T细胞系MT-2和MT-4在中性糖脂和神经节苷脂方面表现出相似的模式。MT-2和MT-4细胞的中性糖脂（葡萄糖神经酰胺和乳糖神经酰胺）明显减少，与其他细胞系相比，它们的一种神经节苷脂GM3仅减少到痕量。MT-4和MT-4/HIV的神经节苷脂在Iatrobeads柱上进一步分离，并通过甲基化和液相二次离子质谱分析鉴定为GM2、GM1a和GD1a。由于与HTLV-I阴性细胞相比，MT-2和MT-4的中性糖脂和神经节苷脂模式独特，因此表明这些变化与HTLV-1感染有关。在感染HIV和未感染HIV的细胞系之间未观察到神经节苷脂组成的显著差异。但值得注意的是，与亲本细胞系相比，Jurkat/HIV和MT-4/HIV细胞中脱唾液酸GM2的含量增加。
