Jun L, Mei Z, Yuan C
Department of Biochemistry, First Medical College of PLA, Guang Zhou, P.R.C.
Int J Immunopharmacol. 1993 Apr;15(3):429-33. doi: 10.1016/0192-0561(93)90055-4.
Using a luminol-dependent, chemiluminescence assay we found tert-butylhydroperoxide to be a strong inhibitor of the respiratory burst of mouse peritoneal macrophages. However, the inhibition of respiratory burst induced by tert-butylhydroperoxide could be prevented after the interperitoneal injection of polysaccharide from Coriolus versicolor (PSK). Further investigation showed that glutathione peroxidase activity was markedly elevated in PSK-treated macrophages. After incubation with tert-butylhydroperoxide, higher activity of glutathione peroxidase was maintained in PSK-treated macrophages. These results suggest that the immunological function of macrophages is related to the activity of glutathione peroxidase. The non-specific immunopolysaccharide might protect macrophages from the damage induced by reactive oxygen species by enhancing antioxidative capacity.
通过使用一种依赖鲁米诺的化学发光测定法，我们发现叔丁基过氧化氢是小鼠腹腔巨噬细胞呼吸爆发的强效抑制剂。然而，在腹腔注射云芝多糖（PSK）后，叔丁基过氧化氢诱导的呼吸爆发抑制作用可被阻止。进一步研究表明，在PSK处理的巨噬细胞中谷胱甘肽过氧化物酶活性显著升高。在用叔丁基过氧化氢孵育后，PSK处理的巨噬细胞中谷胱甘肽过氧化物酶仍保持较高活性。这些结果表明巨噬细胞的免疫功能与谷胱甘肽过氧化物酶的活性有关。这种非特异性免疫多糖可能通过增强抗氧化能力来保护巨噬细胞免受活性氧诱导的损伤。
