Rémond D, Poncet C, Lefaivre J
Station de Recherche sur la Nutrition des Herbivores, Institut National de la Recherche, Agronomique de Clermont-Ferrand, St Genès-Champanelle, France.
J Anim Sci. 1993 May;71(5):1276-80. doi: 10.2527/1993.7151276x.
Eight wethers were used to test the technique. Silicone rubber catheters were introduced into both ruminal veins so that their tips lay a few centimeters from the splenic vein. Arterial blood flow to the rumen was measured by an ultrasonic transit-time flow meter with 3-mm probes implanted around the left and right ruminal arteries. No loss of patency of the venous catheters was observed before slaughter (2 to 6 mo after surgery). There was no evidence of extensive vascular trauma due to catheterization at postmortem examination. In vivo calibration of the flow probes showed that reliable measurements could be made until at least 6 mo after implantation. With an accurate method of blood flow measurement in ruminal arteries and guaranteed long-term catheter patency, it would be possible to make reliable estimates of nutrient uptake across the ruminal wall of sheep over an experimental period of several months.
选用八只阉羊来测试该技术。将硅橡胶导管插入两条瘤胃静脉，使其尖端距离脾静脉几厘米。通过超声渡越时间流量计测量瘤胃的动脉血流量，该流量计配有植入左右瘤胃动脉周围的3毫米探头。屠宰前（手术后2至6个月）未观察到静脉导管通畅性丧失。死后检查未发现因插管导致广泛血管损伤的证据。流量探头的体内校准表明，至少在植入后6个月内可以进行可靠的测量。有了准确测量瘤胃动脉血流量的方法以及保证长期的导管通畅性，就有可能在几个月的实验期内对绵羊瘤胃壁的营养物质吸收进行可靠的估计。
