Predicting amino acid adequacy of diets fed to Holstein steers.

The Cornell Net Carbohydrate and Protein System (CNCPS) and NRC (1985) models were evaluated for accuracy in predicting metabolizable protein (MP) and essential amino acid (EAA) allowable ADG, using chemical body and feed composition data from feeding trials with Holstein steers. Nine Holstein steers (113 to 200 kg) were slaughtered and determined to have the following whole-body essential amino acid composition of (grams/100 grams of protein): arginine, 5.94; histidine, 2.07; isoleucine, 2.28; leucine, 5.72; lysine, 5.81; methionine, 1.99; phenylalanine, 3.04; threonine, 3.52; tryptophan, .57; and valine, 3.32. The NRC and CNCPS were then tested against data from 25 feeding periods, each representing the 56-d growth of 10 Holstein steers (mean BW of 162 kg), to determine their ability to predict the gain allowed by the supply of MP and the first-limiting EAA. The NRC (1985) system accounted for 46% of the variation in MP allowable gain, with an average bias of -30%. The CNCPS accounted for 87 and 73% of the variation in MP and EAA allowable gain, with a bias of 8 and 5%, respectively. The bias was reduced to 3% (R2 of .82) when ADG was predicted by the factor (ME, MP, or EAA) first-limiting ADG.