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综述:转基因动物的分子生物学

Review: molecular biology of transgenic animals.

作者信息

Boyd A L, Samid D

机构信息

Department of Biology, Hood College, Frederick, MD 21701.

出版信息

J Anim Sci. 1993;71 Suppl 3:1-9. doi: 10.2527/1993.71suppl_31x.

Abstract

The amazing pace of discovery within the field of molecular biology has expanded the understanding of as well as the potential for genetic engineering in laboratory and domestic animals. Recently, specific techniques have been perfected, including recombinant DNA, genetic cloning, analysis of gene expression, and the sequencing of nucleic acids and proteins. These molecular techniques combined with methods allowing transfer of the recombinant genes into living cells and fertilized eggs advent the production of transgenic mice and other animals, the application of which is unlimited.

摘要

分子生物学领域惊人的发现速度拓展了人们对实验室动物和家畜基因工程的理解以及其潜力。最近,特定技术已臻完善,包括重组DNA、基因克隆、基因表达分析以及核酸和蛋白质测序。这些分子技术与能将重组基因导入活细胞和受精卵的方法相结合,促成了转基因小鼠及其他动物的产生,其应用前景无限。

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