d'Albis A, Anger M, Lompré A M
Laboratoire de Biologie Physicochimique, URA, CNRS 1131, Université Paris-Sud, Orsay, France.
FEBS Lett. 1993 Jun 14;324(2):178-80. doi: 10.1016/0014-5793(93)81388-g.
The presence of myosin alpha heavy chain in the rabbit masseter has been previously suggested at the protein level [(1991) Basic App. Myol. 1, 23-34; (1991) Histochem. J. 23, 160-170]. To confirm this finding, we cloned most of the mRNA corresponding to the myosin heavy chain S2 subfragment. PCR analysis and subsequent nucleotide sequence determination of the amplified cDNA demonstrates the presence of a myosin alpha heavy chain mRNA in rabbit masticatory muscles.
先前在蛋白质水平上就已表明,兔咬肌中存在肌球蛋白α重链[(1991年)《基础与应用肌学》1, 23 - 34;(1991年)《组织化学杂志》23, 160 - 170]。为证实这一发现,我们克隆了大部分与肌球蛋白重链S2亚片段相对应的mRNA。对扩增的cDNA进行PCR分析及随后的核苷酸序列测定,结果表明兔咀嚼肌中存在肌球蛋白α重链mRNA。