[基于Ad5基因组构建一种无缺陷腺病毒载体，其E3区被带有卡那霉素抗性基因的质粒DNA取代]
[Preparation of a non-defective adenovirus vector based on the Ad5 genome with the E3 region replaced with plasmid DNA with a kanamycin- resistance gene].
作者信息
Doronin K K, Krugliak V A, Grinenko N F, Progonova E V, Grodnitskaia N A, Naroditskiĭ B S, Tikhonenko T I
出版信息
Mol Gen Mikrobiol Virusol. 1993 Jan-Feb(1):31-5.
The plasmid pH5KV14 containing the right end of the Ad5 viral genome (45-100 map units) with deleted nonessential E3 region (78.5-84 map units) has been constructed. A helper-independent recombinant virus Ad5KV14 has been obtained as a result of in vivo transformation of the line 293 human cells by the pH5KV14 plasmid DNA and an EcoRI-A DNA fragment of the virus Ad5 and subsequent recombination. A nondefective recombinant virus Ad5KV17 containing the Ad5 genome with the E3 region substituted for the plasmid DNA fragment coding for the kanamycin-resistance has been constructed by a similar procedure.
已构建出质粒pH5KV14，其含有腺病毒5型（Ad5）病毒基因组的右端（45 - 100个图距单位），且非必需的E3区（78.5 - 84个图距单位）缺失。通过用pH5KV14质粒DNA和Ad5病毒的EcoRI - A DNA片段对293人细胞系进行体内转化并随后进行重组，获得了一种无需辅助病毒的重组病毒Ad5KV14。通过类似的程序构建了一种无缺陷的重组病毒Ad5KV17，其含有用编码卡那霉素抗性的质粒DNA片段替代E3区的Ad5基因组。
Zotero 插件