Fenton B, Glover D M
Department of Anatomy and Physiology, University of Dundee, UK.
Nature. 1993 Jun 17;363(6430):637-40. doi: 10.1038/363637a0.
Mutations in the Drosophila gene polo cause abnormal mitotic and meiotic divisions. This gene encodes a 577-amino-acid protein that has an N-terminal putative kinase domain and a 300-residue C-terminal domain. In budding yeast, a homologous kinase is encoded by CDC5 (ref. 3), a gene required for nuclear division late in the mitotic cycle and during meiosis. Murine homologues have also been described. Here we show that the polo gene product immunoprecipitated from extracts of single Drosophila embryos can phosphorylate casein in vitro, and that the kinase activity peaks cyclically at late anaphase/telophase. This contrasts with the cyclical activity of cyclin B-associated p34cdc2 kinase, which is maximal upon entry into mitosis during the rapid cycles of mitosis in the syncytium.
果蝇polo基因的突变会导致有丝分裂和减数分裂异常。该基因编码一种577个氨基酸的蛋白质，其N端有一个假定的激酶结构域，C端有一个300个残基的结构域。在芽殖酵母中，同源激酶由CDC5基因编码（参考文献3），该基因在有丝分裂周期后期和减数分裂期间的核分裂中是必需的。也已描述了小鼠同源物。在这里，我们表明从单个果蝇胚胎提取物中免疫沉淀的polo基因产物在体外可以磷酸化酪蛋白，并且激酶活性在后期/末期晚期周期性地达到峰值。这与细胞周期蛋白B相关的p34cdc2激酶的周期性活性形成对比，后者在合胞体中有丝分裂的快速周期中进入有丝分裂时达到最大值。
