Skopek R J, Liljemark W F, Bloomquist C G, Rudney J D
Department of Diagnostic and Surgical Sciences, University of Minnesota, School of Dentistry, Minneapolis.
Oral Microbiol Immunol. 1993 Feb;8(1):16-23. doi: 10.1111/j.1399-302x.1993.tb00537.x.
Coaggregations between bacterial species have been widely studied in vitro but not in the mouth. A new in vivo assay was used to measure the rate and composition of indigenous plaque formation onto bovine enamel chips covered with a continuous layer of bacteria. Chips were covered with Streptococcus oralis ATCC 10557, which coaggregated with many oral species, or Streptococcus gordonii S7, which did not coaggregate with these oral species, and placed in the mouth for 4 and 24 h. There were no differences in the number of most indigenous bacterial species isolated from the two streptococcal surfaces. However, the number of Actinomyces viscosus as a proportion of total Actinomyces spp. was significantly different on the two surfaces at 24 h. With the exception of Actinomyces naeslundii and A. viscosus removed from the S7 surface, all indigenous species increased significantly in number from 4 to 24 h, irrespective of the streptococcal surface. This study demonstrated that interbacterial coaggregation had only a limited effect on in vivo plaque development. Thus suggesting that environmental factors, growth or other adherence phenomena are dominant in in vivo plaque formation.
细菌种间的共聚作用已在体外得到广泛研究，但尚未在口腔中进行研究。一种新的体内检测方法被用于测量在覆盖有连续细菌层的牛牙釉质芯片上形成天然菌斑的速率和组成。芯片分别覆盖与多种口腔菌种发生共聚作用的口腔链球菌ATCC 10557或不与这些口腔菌种发生共聚作用的戈登链球菌S7，然后置于口腔中4小时和24小时。从这两种链球菌表面分离出的大多数天然细菌种类的数量没有差异。然而，在24小时时，黏性放线菌在总放线菌属中所占比例在两种表面上存在显著差异。除了从S7表面分离出的内氏放线菌和黏性放线菌外，所有天然菌种的数量从4小时到24小时均显著增加，与链球菌表面无关。这项研究表明，细菌间的共聚作用对体内菌斑形成的影响有限。因此表明环境因素、生长或其他黏附现象在体内菌斑形成中占主导地位。
