Liljemark W F, Bloomquist C G, Bandt C L, Pihlstrom B L, Hinrichs J E, Wolff L F
Department of Diagnostic and Surgical Sciences, University of Minnesota School of Dentistry, Minneapolis.
Oral Microbiol Immunol. 1993 Feb;8(1):5-15. doi: 10.1111/j.1399-302x.1993.tb00536.x.
The distribution of Actinomyces naeslundii, Actinomyces viscosus and Actinomyces odontolyticus in healthy and diseased adult populations was studied in 3 different ways. First, supragingival plaque formation at 2 through 72 h was examined in 12 periodontally healthy adults using a removable pre-measured surface of enamel bonded to molars and premolars. Second, a cross-sectional examination of the composition of both supragingival and subgingival plaque of unknown age was conducted in 205 adults exhibiting periodontal health to moderate disease. Third, the effects of oral hygiene instruction and root planing on the subgingival microflora of a subset of 19 subjects with moderate periodontitis were examined. The evaluation of 12 adults revealed that the predominant species in early plaque formation (2, 4 and 8 h) was A. odontolyticus. A. viscosus and A. naeslundii were present in developing plaques in almost all subjects in 2-h plaque, but absent in half the subjects when 4-, 8- or 24-h plaque was examined. These two species significantly increased in numbers per mm2 enamel surface area in 48- and 72-h plaques. A. odontolyticus was not related to clinical signs of periodontal disease in 205 adults, and its subgingival proportions in plaque did not change following periodontal treatment of 19 individuals. A. naeslundii was found in significantly higher numbers in supragingival than subgingival plaques in the 205 adults examined. The mean proportion of A. naeslundii significantly decreased as the magnitude of probing depth and attachment loss increased. The proportions of A. naeslundii and A. viscosus were found to be significantly increased in subgingival plaques following periodontal treatment.
采用3种不同方法研究了内氏放线菌、黏性放线菌和溶牙放线菌在健康和患病成年人群中的分布情况。第一，在12名牙周健康的成年人中，使用与磨牙和前磨牙相连的可移动预测量釉质表面，检测2至72小时内龈上菌斑的形成情况。第二，对205名牙周健康至中度患病的成年人进行横断面检查，分析年龄未知的龈上和龈下菌斑的组成。第三，研究口腔卫生指导和根面平整对19名中度牙周炎患者龈下微生物群的影响。对12名成年人的评估显示，早期菌斑形成（2、4和8小时）中的主要菌种是溶牙放线菌。在2小时菌斑中，几乎所有受试者的发育中菌斑都存在黏性放线菌和内氏放线菌，但在检查4、8或24小时菌斑时，半数受试者中不存在这两种菌。在48和72小时菌斑中，这两种菌每平方毫米釉质表面积的数量显著增加。在205名成年人中，溶牙放线菌与牙周病的临床症状无关，19名个体接受牙周治疗后，其在龈下菌斑中的比例没有变化。在所检查的205名成年人中，龈上菌斑中内氏放线菌的数量明显高于龈下菌斑。随着探诊深度和附着丧失程度的增加，内氏放线菌的平均比例显著下降。牙周治疗后，龈下菌斑中内氏放线菌和黏性放线菌的比例显著增加。
