Eipper B A, Bloomquist B T, Husten E J, Milgram S L, Mains R E
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Ann N Y Acad Sci. 1993 May 31;680:147-60. doi: 10.1111/j.1749-6632.1993.tb19681.x.
Studies on the mRNAs encoding PAM and on the various PAM proteins have begun to reveal some of the intricate mechanisms used to optimize the ability of this enzyme to carry out the alpha-amidation of peptides. Comparison of the regulatory elements governing expression of the various enzymes involved in peptide processing should reveal common elements. Knowledge of the processing enzymes themselves should help us to understand how these enzymes function in the secretory granule environment. In addition to their catalytic domains, other processing enzymes, like PAM, may well have processing domains and routing domains designed to optimize their ability to function in secretory granules.
对编码PAM的mRNA以及各种PAM蛋白的研究已开始揭示一些用于优化该酶进行肽α-酰胺化能力的复杂机制。对参与肽加工的各种酶的表达调控元件进行比较，应能揭示共同元件。对加工酶本身的了解应有助于我们理解这些酶在分泌颗粒环境中的功能。除了其催化结构域，其他加工酶，如PAM，很可能具有旨在优化其在分泌颗粒中功能能力的加工结构域和转运结构域。
