Use of a combined synthetic fibre bag method to estimate the true total tract digestion of organic matter and nitrogen of hay and grass silage in cows.

A 4 x 4 Latin square experiment was designed to determine and compare the total tract digestion of organic matter (OM) and nitrogen of hay and grass silage by combining the ruminal and intestinal digestion estimates obtained by the rumen bag and mobile bag methods. The diets were composed of forage alone or 30% forage and 70% barley. The rumen degradation (RD) was calculated using the degradation constants a, b, and c obtained to the forages, according to the equation RD = a+bc/(c+k). Intestinal digestion of escape feeds was estimated using the feed residues that resisted rumen degradation during 16 hours. For comparative purposes, the total tract digestion was also calculated based on the actual rumen degradation of forages during 16 hours rumen incubation. The effect of dietary interaction on the digestion of forages was assessed. Rumen degradation values obtained by 16 hours incubation did not provide sufficient basis to estimate reliably the total tract digestion by the combined bag method. Using calculated RD total tract digestion of OM of hay and grass silage were rather close to conventional in vivo digestion coefficient of respective feeds similar in chemical composition, when barley was not included in the diet. With hay, but not with grass silage, barley always markedly reduced the rumen degradation and total digestion of OM. Barley supplementation always increased the proportion of feed digested in the intestine. Nitrogen digestion was always higher with grass silage than with hay, and the combined bag method produced N digestion values 17.6-22.0%-units higher than those in the feed table. Barley supplementation did not affect the N digestion of the forages.(ABSTRACT TRUNCATED AT 250 WORDS)