Elefanty A G, Cory S
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Biotechniques. 1993 May;14(5):770-4.
A simple method is described for rapidly screening fibroblast packaging cell line clones for high-titer secretion of retroviruses carrying a selectable marker. Virus-containing supernatants are used to infect FDC-P1 myeloid cells in 24-well tissue culture plates, selection is applied and wells containing live cells (infected by retrovirus and thus expressing the selectable marker) are detected after 5 to 10 days. The number of live cells in each well is proportional to the retroviral titer of the infecting supernatant. The assay is quick to set up and allows simultaneous screening of many samples.
本文描述了一种简单的方法，用于快速筛选成纤维细胞包装细胞系克隆，以获得携带选择标记的逆转录病毒的高滴度分泌。含病毒的上清液用于感染24孔组织培养板中的FDC-P1髓样细胞，进行选择，并在5至10天后检测含有活细胞（被逆转录病毒感染并因此表达选择标记）的孔。每个孔中的活细胞数量与感染上清液的逆转录病毒滴度成正比。该检测方法易于设置，并且允许同时筛选多个样品。
