Ferguson J A, Reeves W C, Hardy J L
Am J Vet Res. 1977 Apr;38(4):425-30.
Serologic studies in 24 ponies indicated that prevaccination antibodies to Venezuelan equine encephalomyelitis (VEE) virus (strain TC-83) had no influence on hemagglutination-inhibition (HI) antibody stimulation by western equine encephalomyelitis (WEE) or eastern equine encephalomyelits (EEE)-WEE vaccines. However, studies of the effects of VEE neutralizing antibodies on neutralizing antibody stimulation by the heterologous alphavirus vaccines were inconclusive. The VEE, WEE, and EEE antibody responses were studied in 18 VEE-vaccinated (strain TC-83) animals (13 ponies and 5 horses) at 9 to 14 months after challenge of immunity with virulent VEE virus. The VEE HI, and neutralizing antibodies in combination were associated with greater suppression of VEE antibody stimulation than were VEE neutralizing antibodies alone. The VEE geometric mean antibody titers (GMT) increased 26- and 64-fold for HI antibodies and for neutralizing antibodies, respectively, in 5 animals that had prechallenge exposure titers of VEE neutralizing antibodies only. In contrast, the GMT increased only threefold and eightfold for VEE HI antibodies and for neutralizing antibodies, respectively, in 8 animals that had prechallenge exposure titers of both VEE HI and neutralizing antibodies. Five animals which were seronegative for prechallenge VEE antibodies and had survived challenge exposure gave primary VEE-immune response. Protection in these animals may have resulted from high-titer cross-reacting antibodies to WEE or EEE viruses, or both. Changes in the GMT for WEE HI, neutralizing, and complement-fixation antibodies after challenge exposure were statistically insignificant. However, a statistically significant increase in the EEE HI-GMT occurred in ponies that had low (or no) prechallenge exposure titers of VEE antibodies.
对24匹小马进行的血清学研究表明，接种委内瑞拉马脑炎（VEE）病毒（TC - 83株）疫苗前的抗体，对西部马脑炎（WEE）或东部马脑炎（EEE）-WEE疫苗刺激血凝抑制（HI）抗体没有影响。然而，关于VEE中和抗体对异源甲病毒疫苗刺激中和抗体的影响的研究尚无定论。在用强毒VEE病毒激发免疫9至14个月后，对18只接种VEE（TC - 83株）疫苗的动物（13匹小马和5匹马）的VEE、WEE和EEE抗体反应进行了研究。与单独的VEE中和抗体相比，VEE HI和中和抗体联合起来对VEE抗体刺激的抑制作用更强。在5只仅在激发前有VEE中和抗体暴露滴度的动物中，VEE几何平均抗体滴度（GMT）对于HI抗体分别增加了26倍，对于中和抗体分别增加了64倍。相比之下，在8只激发前既有VEE HI抗体又有中和抗体暴露滴度的动物中，VEE HI抗体和中和抗体的GMT分别仅增加了3倍和8倍。5只激发前VEE抗体血清阴性且在激发暴露中存活下来的动物产生了原发性VEE免疫反应。这些动物的保护作用可能是由于对WEE或EEE病毒或两者的高滴度交叉反应抗体所致。激发暴露后，WEE HI、中和及补体结合抗体的GMT变化在统计学上无显著意义。然而，在激发前VEE抗体暴露滴度低（或无）的小马中，EEE HI - GMT出现了统计学上的显著增加。
