Kai K, Tateyama S, Miyoshi N, Yamaguchi R, Uchida K, Rostami M
Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Japan.
J Vet Med Sci. 1993 Apr;55(2):319-21. doi: 10.1292/jvms.55.319.
Genomic DNAs of cattle, horses, pigs, dogs, cats and chickens were surveyed using Southern blot hybridization analysis, with a human EGFR cDNA fragment. Several bands with different numbers and molecular weights were observed under the condition of low stringency in the individual animal species. The bands showing DNA polymorphism were observed among bovine genomic PstI-digested DNAs from 4 individuals and EcoRI-digested genomic DNAs from 4 chickens. These results may provide basic data which are useful for analysis of tumorigenetic mechanisms in domestic animals.
使用人表皮生长因子受体（EGFR）cDNA片段，通过Southern杂交分析对牛、马、猪、狗、猫和鸡的基因组DNA进行了检测。在低严谨条件下，在各个动物物种中观察到了几条数量和分子量不同的条带。在4头奶牛的基因组PstI酶切DNA和4只鸡的EcoRI酶切基因组DNA中观察到了显示DNA多态性的条带。这些结果可能为分析家畜肿瘤发生机制提供有用的基础数据。
