Griffiths W C, Dextraze P, Diamond I
Ann Clin Lab Sci. 1977 Mar-Apr;7(2):141-5.
A radioimmunoassay method for the determination of combined gentamicin isomers in serum has been adapted and tested. The procedure uses tritiated gentamicin, rabbit antiserum against a gentamicin-human albumin conjugate, and dextran-charcoal separation. The day-to-day coefficient of variation is 6 percent, and frozen samples are stable for at least one month. There was no cross reactivity of the antiserum with any of several tested antibiotics or with any of some tested commonly used non-antibiotic hospital pharmacy preparations. The results from the procedure correlated well with those of an enzymatic radioacetylation technique and, except for a significant incidence of "outliers", with a microbiological assay. As expected, patient serum values, both maximum and minimum, showed no correlation with dose size.
一种用于测定血清中庆大霉素结合异构体的放射免疫分析方法已被调整并进行了测试。该方法使用氚标记的庆大霉素、抗庆大霉素-人白蛋白偶联物的兔抗血清以及葡聚糖-活性炭分离法。日常变异系数为6%，冷冻样本至少稳定一个月。该抗血清与几种测试抗生素中的任何一种以及一些测试的常用医院药房非抗生素制剂均无交叉反应。该方法的结果与酶促放射性乙酰化技术的结果相关性良好，并且除了有显著的“异常值”发生率外，与微生物测定法的结果也相关性良好。正如预期的那样，患者血清值的最大值和最小值均与剂量大小无关。
