Boix J, Roca J
Department of Physiological Sciences, University of Barcelona School of Medicine, Spain.
Cytometry. 1993;14(4):465-7. doi: 10.1002/cyto.990140418.
By application of minor variations in the usual method of centrifugal elutriation, we have been able to separate a nuclear suspension from rooster testes. We have obtained a population of elongated or late spermatid nuclei (98% purity), a population of round or early spermatid nuclei (90% purity), and, finally, a mixed population of meiotic and pre-meiotic nuclei.