在杆状病毒感染的昆虫细胞中高效生产生物活性人胞质磷脂酶A2
High-level production of biologically active human cytosolic phospholipase A2 in baculovirus-infected insect cells.
作者信息
Amegadzie B Y, Jiampetti D, Craig R J, Appelbaum E, Shatzman A R, Mayer R J, DiLella A G
机构信息
Department of Gene Expression Sciences, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939.
出版信息
Gene. 1993 Jun 30;128(2):307-8. doi: 10.1016/0378-1119(93)90580-v.
PMID:8514199
Abstract
Infection of Spodoptera frugiperda insect cells with a recombinant baculovirus expressing human cytosolic phospholipase A2 (cPLA2) resulted in the production of biologically active protein. The level of recombinant human cPLA2 production in infected insect cells was at least 50-fold higher than that observed in human monoblast U937 cells.
摘要
用表达人胞质型磷脂酶A2（cPLA2）的重组杆状病毒感染草地贪夜蛾昆虫细胞，可产生具有生物活性的蛋白质。感染昆虫细胞中重组人cPLA2的产量比人单核细胞U937细胞中观察到的产量至少高50倍。
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