Amegadzie B Y, Jiampetti D, Craig R J, Appelbaum E, Shatzman A R, Mayer R J, DiLella A G
Department of Gene Expression Sciences, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939.
Gene. 1993 Jun 30;128(2):307-8. doi: 10.1016/0378-1119(93)90580-v.
Infection of Spodoptera frugiperda insect cells with a recombinant baculovirus expressing human cytosolic phospholipase A2 (cPLA2) resulted in the production of biologically active protein. The level of recombinant human cPLA2 production in infected insect cells was at least 50-fold higher than that observed in human monoblast U937 cells.
用表达人胞质型磷脂酶A2（cPLA2）的重组杆状病毒感染草地贪夜蛾昆虫细胞，可产生具有生物活性的蛋白质。感染昆虫细胞中重组人cPLA2的产量比人单核细胞U937细胞中观察到的产量至少高50倍。
