Bushart G B, Vetter U, Hartmann W
Abteilungen für Paediatrie I und II, Universität Ulm, Germany.
Horm Metab Res. 1993 Apr;25(4):210-3. doi: 10.1055/s-2007-1002078.
Hexose uptake of the transformed lymphocyte cell line IM9 was analysed with respect to kinetics, responsiveness to insulin and insulin-like growth factor I and to cell cycle dependency. We found a Km of 0.76 mM and a Vmax of 14.9 nMol/min/mg protein for 2-deoxy-D-[3H]glucose uptake. This Km is in accordance with the values described for the erythrocyte type and the brain type glucose transporter. Hexose uptake of IM9 lymphocytes was not altered by physiological concentrations of insulin or IGF I. We investigated the 2-deoxy-D-[3H]glucose transport in cells, enriched in the different cell cycle phases by counterflow elutriation. The results were calculated for hypothetical pure fractions. Hexose uptake per cell is more than doubled during the S-phase and returns in G2-cells to the basal value of G1-phase.
我们分析了转化淋巴细胞系IM9的己糖摄取情况，包括动力学、对胰岛素和胰岛素样生长因子I的反应性以及对细胞周期的依赖性。我们发现2-脱氧-D-[3H]葡萄糖摄取的米氏常数（Km）为0.76 mM，最大反应速度（Vmax）为14.9 nmol/分钟/毫克蛋白。这个Km值与红细胞型和脑型葡萄糖转运体所描述的值一致。IM9淋巴细胞的己糖摄取不受生理浓度胰岛素或胰岛素样生长因子I的影响。我们通过逆流淘析研究了富集于不同细胞周期阶段的细胞中的2-脱氧-D-[3H]葡萄糖转运。结果是针对假设的纯组分计算得出的。每个细胞的己糖摄取在S期增加了一倍多，并在G2期细胞中恢复到G1期的基础值。
