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大鼠垂体中卵泡抑素信使核糖核酸水平的调节

Regulation of follistatin messenger ribonucleic acid levels in the rat pituitary.

作者信息

Kaiser U B, Chin W W

机构信息

Department of Medicine, Brigham and Women's Hospital, Howard Hughes Medical Institute, Boston, Massachusetts 02115.

出版信息

J Clin Invest. 1993 Jun;91(6):2523-31. doi: 10.1172/JCI116488.

DOI:10.1172/JCI116488
PMID:8514864
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC443313/
Abstract

Follistatin is a glycoprotein, originally isolated from the gonads, that specifically inhibits follicle-stimulating hormone (FSH) biosynthesis and secretion. We have previously detected follistatin mRNA in rat pituitary gonadotropes. To assess the potential physiologic role of follistatin in the rat pituitary, we have investigated the effects of gonadectomy (GDX) and of sex steroid replacement on pituitary follistatin gene expression. Follistatin mRNA levels in individual rat pituitaries were measured by a quantitative reverse transcription-polymerase chain reaction assay. Female and male rats 21 d old underwent surgical GDX and were then killed 21 d after GDX. Follistatin mRNA levels in rat pituitary increased 3.2 +/- 1.5-fold (P < 0.01) in GDX female rats and 8.2 +/- 2.0-fold (P < 0.005) in GDX male rats, compared with intact female and male controls, respectively. Replacement therapy with 17 beta-estradiol-3-benzoate (10 micrograms/100 g body weight) subcutaneously daily for 7 d in GDX female rats resulted in a slight further increase in follistatin mRNA levels compared to GDX females. In contrast, therapy with testosterone propionate (500 micrograms/100 g body weight) subcutaneously daily for 7 d in GDX male rats resulted in a decrease in follistatin mRNA levels, towards but not completely back to baseline levels in intact males. Time-course studies in adult male and female rats showed that the increase in follistatin mRNA levels after GDX is rapid, with significant increases occurring within 24 h after GDX, and parallels or precedes increases in FSH beta mRNA levels and FSH secretion. The regulation of follistatin mRNA levels in the rat pituitary by GDX and by sex steroids suggests that follistatin may be important as an autocrine or paracrine factor in the regulation of FSH.

摘要

卵泡抑素是一种糖蛋白，最初从性腺中分离出来，它能特异性抑制促卵泡激素（FSH）的生物合成和分泌。我们之前在大鼠垂体促性腺细胞中检测到了卵泡抑素mRNA。为了评估卵泡抑素在大鼠垂体中的潜在生理作用，我们研究了性腺切除（GDX）和性类固醇替代对垂体卵泡抑素基因表达的影响。通过定量逆转录-聚合酶链反应测定法测量个体大鼠垂体中的卵泡抑素mRNA水平。21日龄的雌性和雄性大鼠接受手术性腺切除，然后在性腺切除后21天处死。与完整的雌性和雄性对照相比，性腺切除的雌性大鼠垂体中卵泡抑素mRNA水平增加了3.2±1.5倍（P<0.01），性腺切除的雄性大鼠中增加了8.2±2.0倍（P<0.005）。在性腺切除的雌性大鼠中，每天皮下注射17β-雌二醇-3-苯甲酸酯（10微克/100克体重）进行7天的替代治疗，与性腺切除的雌性大鼠相比，卵泡抑素mRNA水平略有进一步升高。相反，在性腺切除的雄性大鼠中，每天皮下注射丙酸睾酮（500微克/100克体重）进行7天的治疗，导致卵泡抑素mRNA水平下降，接近但未完全恢复到完整雄性大鼠的基线水平。成年雄性和雌性大鼠的时间进程研究表明，性腺切除后卵泡抑素mRNA水平迅速升高，在性腺切除后24小时内显著升高，与FSHβmRNA水平和FSH分泌的升高平行或先于其升高。性腺切除和性类固醇对大鼠垂体中卵泡抑素mRNA水平的调节表明，卵泡抑素作为一种自分泌或旁分泌因子在FSH的调节中可能很重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e123/443313/a119fbb5056f/jcinvest00055-0199-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e123/443313/e908c386e1f0/jcinvest00055-0195-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e123/443313/e908c386e1f0/jcinvest00055-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e123/443313/49dbb0743461/jcinvest00055-0195-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e123/443313/237fbf743b1a/jcinvest00055-0195-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e123/443313/b22d087815cb/jcinvest00055-0195-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e123/443313/8bbcac2192b3/jcinvest00055-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e123/443313/a119fbb5056f/jcinvest00055-0199-b.jpg

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