Melchior D L, Steim J M
Biochim Biophys Acta. 1977 Apr 1;466(1):148-59. doi: 10.1016/0005-2736(77)90215-2.
The temperature-dependent pattern of incorporation of palmitate and oleate from the growth medium into Acholeplasma laidlawii membrane lipids correlates with the physical state of the membrane defined by calorimetry. Both the pattern and the state can be changed at will by changing the fatty acid composition of the membrane lipids. The ratio of palmitate to oleate incorporated is independent of temperature when the membrane bilayer is below its transition and fully ordered, but becomes temperature dependent upon the onset of the transition and continues to be temperature dependent when the membrane is above its transition and fully fluid. This behavior is mimicked by the physical binding of palmitate and oleate to bilayers of extracted membrane lipids and to bilayers of lecithin. Selective binding by membranes may provide a means for controlling lipid fatty acid composition without invoking an enzymatic mechanism.
从生长培养基中摄取的棕榈酸酯和油酸酯掺入莱氏无胆甾原体膜脂的温度依赖性模式，与通过量热法定义的膜的物理状态相关。通过改变膜脂的脂肪酸组成，模式和状态都可以随意改变。当膜双层低于其转变温度并完全有序时，掺入的棕榈酸酯与油酸酯的比例与温度无关，但在转变开始时变得依赖于温度，并且当膜高于其转变温度并完全呈流体状态时继续依赖于温度。棕榈酸酯和油酸酯与提取的膜脂双层以及卵磷脂双层的物理结合模拟了这种行为。膜的选择性结合可能提供一种在不涉及酶机制的情况下控制脂质脂肪酸组成的方法。
