Chernos V I, Vovk T S, Ivanova O N, Antonova T P, Loparev V N
Mol Gen Mikrobiol Virusol. 1993 Mar-Apr(2):30-4.
The integrative plasmids with the expressive marker gene for beta-galactosidase were constructed for insertional inactivation of nonessential genes E7R and D8L of vaccinia virus located in the central region of the viral genome. Inactivation of the D8L gene in the strains WR and LIVP results in smaller viral plaques in the culture of chicken embryo cells, decreases the viral ability to propagate in mouse brain and has no effect on the size and character of damage in intracutaneous infection of rabbits. Inactivation of E7R gene did not affect the known biological properties of the virus. The existence of the nonessential genes in the central region of the viral genome, inactivation of which does not result in viral attenuation, can be used for increase of antigenic activity of the live attenuated vaccines.
构建了带有β-半乳糖苷酶表达标记基因的整合质粒，用于插入失活痘苗病毒位于病毒基因组中心区域的非必需基因E7R和D8L。在WR和LIVP毒株中使D8L基因失活，会导致鸡胚细胞培养物中的病毒蚀斑变小，降低病毒在小鼠脑中的增殖能力，并且对兔皮内感染的损伤大小和特征没有影响。使E7R基因失活不影响病毒已知的生物学特性。病毒基因组中心区域存在非必需基因，使其失活不会导致病毒减毒，可用于提高减毒活疫苗的抗原活性。
