[来自毛云芝的漆酶——免疫酶分析的一种新标记酶]

[Laccase from Coriolus hirsutus--a new marker enzyme for immunoenzyme analysis].

作者信息

Skorobogat'ko O V, Gindilis A L, Shuster A M, Troitskaia E N, Iaropolov A I

出版信息

Prikl Biokhim Mikrobiol. 1993 May-Jun;29(3):354-61.

Abstract

A new immunochemical reagent is proposed which contains laccase, isolated from the culture liquid of the basidial fungus Coriolus hirsutus, as a marker enzyme. The feasibility of immunolaccase conjugates for different variants of immunoassay, i.e. "sandwich", competitive and indirect, is demonstrated. The comparison of immunolaccase and immunoperoxidase conjugates showed that the absolute sensitivity of laccase-antibody conjugates was 3 times higher than that of antibody-peroxidase conjugates (7.7 x 10(-11) M and 2.3 x 10(-10) M, respectively). The assay based on antibody-laccase conjugates is simpler than that employing antibody-peroxidase conjugates, since in the former case air oxygen in used as the second substrate of the enzymatic reaction.

摘要

提出了一种新的免疫化学试剂，其包含从担子菌毛云芝的培养液中分离得到的漆酶作为标记酶。证明了免疫漆酶缀合物用于不同免疫测定变体（即“夹心”、竞争和间接）的可行性。免疫漆酶和免疫过氧化物酶缀合物的比较表明，漆酶-抗体缀合物的绝对灵敏度比抗体-过氧化物酶缀合物高3倍（分别为7.7×10⁻¹¹ M和2.3×10⁻¹⁰ M）。基于抗体-漆酶缀合物的测定比使用抗体-过氧化物酶缀合物的测定更简单，因为在前一种情况下，空气中的氧气用作酶促反应的第二底物。