小鼠肠道葡萄糖转运蛋白底物依赖性调节的隐窝/绒毛位点
Crypt/villus site of substrate-dependent regulation of mouse intestinal glucose transporters.
作者信息
Ferraris R P, Diamond J M
机构信息
Department of Physiology, University of California Medical Center, Los Angeles 90024-1751.
出版信息
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5868-72. doi: 10.1073/pnas.90.12.5868.
Abstract
The intestinal epithelium is in a constant state of turnover, with cells differentiating at the crypts and then migrating toward the tips of the villi. Does substrate-dependent regulation of intestinal Na+/D-glucose cotransporters occur only in crypt cells, or can transport activity be subsequently reprogrammed in mature enterocytes? We used in situ, glucose-protectable specific phlorizin binding to determine site density of brush border glucose transporters in enterocytes fractionated along the crypt/villus axis of mice that were killed shortly after drastic changes in carbohydrate levels of their diets. Dietary carbohydrate-induced changes in site density of specific phlorizin binding initially appeared only in crypt cells before spreading, over the course of several days, to the villus tips. Thus, only crypt cells perceive the signal for glucose transporter regulation, and the observed time lag of diet-induced changes in intestinal glucose uptake is due mainly to cell migration times.
摘要
肠上皮处于持续更新状态，细胞在隐窝处分化，然后向绒毛顶端迁移。肠道钠/葡萄糖共转运蛋白的底物依赖性调节仅发生在隐窝细胞中，还是在成熟肠上皮细胞中其转运活性随后可被重新编程？我们利用原位葡萄糖可保护的特异性根皮素结合来确定沿饮食碳水化合物水平发生剧烈变化后不久处死的小鼠隐窝/绒毛轴分离的肠上皮细胞中刷状缘葡萄糖转运蛋白的位点密度。饮食碳水化合物诱导的特异性根皮素结合位点密度变化最初仅出现在隐窝细胞中，然后在几天内扩散到绒毛顶端。因此，只有隐窝细胞能感知葡萄糖转运蛋白调节信号，饮食诱导的肠道葡萄糖摄取变化中观察到的时间滞后主要是由于细胞迁移时间。
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Phlorizin as a probe of the small-intestinal Na+,D-glucose cotransporter. A model.根皮苷作为小肠钠-葡萄糖共转运体的探针。一个模型。
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