Toebe C S, Montenegro-James S, Noya O, Riggione F, James M A
Department of Tropical Medicine and Parasitology, Tulane University, New Orleans, Louisiana 70112.
Trop Med Parasitol. 1993 Mar;44(1):1-4.
Indirect fluorescent antibody (IFA) tests and enzyme-linked immunosorbent assays (ELISA) were used to measure antibodies to Plasmodium falciparum in patients with acute malaria from Bolivar State, Venezuela. Antibody titers increased significantly with repeated malarial episodes. IgG antibody responses to 4 synthetic peptides (termed C2, C3, C5, C10) derived from a 70 kDa P. falciparum (Indochina I/CDC strain) exoantigen were evaluated by a peptide-ELISA with overall positivity rates of 20%, 40%, 20% and 58%, respectively. Seropositivity to peptide C10 was consistently over 50% (range 53-75%) among patients of different ages. Overall IgM reactivity to the respective peptides was 53%, 30%, 83% and 70%. IgM reactivity was generally greater in patients with primary malarial infections. The ELISA is a useful adjunct to the IFA in measuring naturally-occurring antibodies to specific parasite proteins.
采用间接荧光抗体（IFA）试验和酶联免疫吸附测定（ELISA）检测委内瑞拉玻利瓦尔州急性疟疾患者体内的恶性疟原虫抗体。抗体滴度随着疟疾病程反复而显著升高。通过肽段ELISA评估了对源自70 kDa恶性疟原虫（印度支那I/疾病预防控制中心株）外抗原的4种合成肽（称为C2、C3、C5、C10）的IgG抗体反应，总体阳性率分别为20%、40%、20%和58%。不同年龄患者中，对肽段C10的血清阳性率始终超过50%（范围为53%-75%）。对相应肽段的总体IgM反应性分别为53%、30%、83%和70%。原发性疟疾感染患者的IgM反应性通常更高。ELISA在检测针对特定寄生虫蛋白的天然抗体方面是IFA的有用辅助手段。
