Uche U E, Jones T W, Boid R
Royal Veterinary College, London, UK.
Trop Med Parasitol. 1993 Mar;44(1):27-31.
The antibody response to Trypanosoma evansi antigens in rabbits experimentally infected with T. evansi was monitored by SDS-PAGE and "Western" blotting. As defined by the number of antigenic components recognised and the intensity with which they were labelled, responses were predominantly IgG, followed by IgM and IgA. During infection and subsequent drug treatment seven major antigenic components (94, 85, 75.5, 67, 60.5, 43, and 28.6 kDa) were identified by IgG. Antibodies to a 32.4 kDa and three other components (30, 28.6 and 12.5 kDa) appeared during infection and after chemotherapy respectively. Three components (94, 67 and 60.5 kDa) recognised by IgG were also detected by IgM and IgA. Furthermore, two bands (30 and 12.5 kDa) were identified by IgM and one (12.5 kDa) by IgA only after drug treatment, while a 28.6 kDa component was recognised by IgM and IgA during the same period. These findings constitute a basis for the exploration of class-specific antibody responses to T. evansi antigens with relevance to diagnosis, protection and pathology.
通过SDS - PAGE和“Western”印迹法监测实验感染伊氏锥虫的家兔对伊氏锥虫抗原的抗体反应。根据识别的抗原成分数量及其标记强度来定义，反应主要为IgG，其次是IgM和IgA。在感染及随后的药物治疗期间，IgG识别出七种主要抗原成分（94、85、75.5、67、60.5、43和28.6 kDa）。在感染期间和化疗后分别出现了针对32.4 kDa和其他三种成分（30、28.6和12.5 kDa）的抗体。IgG识别的三种成分（94、67和60.5 kDa）也被IgM和IgA检测到。此外，仅在药物治疗后，IgM识别出两条带（30和12.5 kDa），IgA识别出一条带（12.5 kDa），而在同一时期，IgM和IgA识别出一个28.6 kDa的成分。这些发现为探索针对伊氏锥虫抗原的类特异性抗体反应奠定了基础，这与诊断、保护和病理学相关。
