de Beer P M, Windt M L, Bouic P J
Department of Urology, University of Stellenbosch, Tygerberg, South-Africa.
Andrologia. 1993 May-Jun;25(3):149-52. doi: 10.1111/j.1439-0272.1993.tb02699.x.
Immunological infertility is thought to be caused by the binding of antibodies to 'fertility-related' antigen(s) on the sperm membrane. We compared antibody profiles in sera from 20 ASA(+) and ASA(-) men, using a sperm membrane extract as an antigen. Antigens were separated by SDS-PAGE under reducing conditions. The patients were classed as ASA(+) by the MAR (> 50%), d-IBT (> 20%) and TAT (> 1:64). The results showed that immunoreactive bands in both the ASA(+) and ASA(-) groups were heterogeneous and included bands covering the whole molecular weight range. Statistical analysis showed significantly more patients in the ASA(+) group having immunoreactive bands at molecular weights of 32 Kd (P = 0.006) and 79 Kd (P = 0.02) when compared to the ASA(-) group. In the ASA(-) group significantly more patients had reactive bands at 81 Kd (P = 0.01) when compared to the ASA(+) group. The 32 Kd antigen reacted only with sera from ASA(+) patients. We conclude that differences exist between the ASA(+) and ASA(-) groups when this extraction method is used and that the isolation and purification of the 32 Kd protein may justify further investigation.
免疫性不育被认为是由抗体与精子膜上的“生育相关”抗原结合所致。我们以精子膜提取物为抗原，比较了20名抗精子抗体阳性（ASA(+)）和抗精子抗体阴性（ASA(-)）男性血清中的抗体谱。抗原在还原条件下通过SDS-PAGE进行分离。通过混合抗球蛋白反应（MAR）（>50%）、直接免疫珠试验（d-IBT）（>20%）和TAT（>1:64）将患者分类为ASA(+)。结果显示，ASA(+)组和ASA(-)组中的免疫反应条带均具有异质性，且包括覆盖整个分子量范围的条带。统计分析表明，与ASA(-)组相比，ASA(+)组中分子量为32 Kd（P = 0.006）和79 Kd（P = 0.02）时具有免疫反应条带的患者明显更多。与ASA(+)组相比，ASA(-)组中分子量为81 Kd时具有反应条带的患者明显更多。32 Kd抗原仅与ASA(+)患者的血清发生反应。我们得出结论，当使用这种提取方法时，ASA(+)组和ASA(-)组之间存在差异，并且32 Kd蛋白的分离和纯化可能值得进一步研究。
