Slupsky J R, Duggan-Keen M, Booth L A, Karpas A, Rhodes E G, Cawley J C, Zuzel M
Department of Haematology, University of Liverpool.
Br J Haematol. 1993 Apr;83(4):567-73. doi: 10.1111/j.1365-2141.1993.tb04692.x.
Plasma cells within bone marrow aspirates from multiple myeloma patients have been shown to be reactive with the lectin peanut agglutinin (PNA). This has been recently exploited by using PNA for purging bone marrow of malignant cells in autotransplantation therapy of the disease. The purpose of this investigation was to isolate and characterize the PNA-binding proteins of myeloma cells. We used the malignant plasma cell-derived line Karpas-620 (K620) as a model, and showed by affinity chromatography, SDS-PAGE, and immunoprecipitation that, among several PNA-binding proteins, a major one is an incompletely sialylated form of CD44. CD44 is a well-known homing receptor protein which is rich in carbohydrate and usually completely sialylated so that it does not react with PNA. We have then examined the PNA reactivity of myeloma cells from different patients and showed a clear difference in the profile of PNA-binding proteins from case to case. Moreover, in contrast to K620 cells, some of the patient plasma cells tested did not have a PNA-binding form of CD44. In conclusion, therefore, we have shown that a number of different proteins participate in PNA binding by malignant plasma cells. Moreover, we have demonstrated a novel, incompletely sialylated form of CD44 on a myeloma cell line. It is known that the level of glycosylation of CD44 and other proteins may affect their function, but how this relates to the malignant behaviour of plasma cells remains to be determined.
多发性骨髓瘤患者骨髓穿刺液中的浆细胞已被证明可与凝集素花生凝集素（PNA）发生反应。最近，在该疾病的自体移植治疗中，人们利用PNA来清除骨髓中的恶性细胞。本研究的目的是分离并鉴定骨髓瘤细胞中与PNA结合的蛋白质。我们以恶性浆细胞衍生系Karpas-620（K620）为模型，通过亲和层析、SDS-PAGE和免疫沉淀法表明，在几种与PNA结合的蛋白质中，主要的一种是CD44的不完全唾液酸化形式。CD44是一种众所周知的归巢受体蛋白，富含碳水化合物，通常完全唾液酸化，因此不与PNA反应。然后我们检测了不同患者骨髓瘤细胞的PNA反应性，结果显示不同病例中PNA结合蛋白的谱存在明显差异。此外，与K620细胞不同，一些检测的患者浆细胞没有PNA结合形式的CD44。因此，总之，我们已表明许多不同的蛋白质参与恶性浆细胞与PNA的结合。此外，我们在骨髓瘤细胞系上发现了一种新的、不完全唾液酸化形式的CD44。已知CD44和其他蛋白质的糖基化水平可能影响其功能，但这与浆细胞的恶性行为有何关系仍有待确定。
