一些非甾体抗炎药的毛细管区带电泳和胶束电动毛细管色谱法

Capillary zone electrophoresis and micellar electrokinetic capillary chromatography of some non-steroidal antiinflammatory drugs (NSAIDs).

作者信息

Donato M G, Van den Eeckhout E, Van den Bossche W, Sandra P

机构信息

Laboratories for Drug Quality Control and Medicinal Chemistry, U.G., Gent, Belgium.

出版信息

J Pharm Biomed Anal. 1993 Mar;11(3):197-201. doi: 10.1016/0731-7085(93)80196-8.

DOI:10.1016/0731-7085(93)80196-8

PMID:8518317

Abstract

The possibilities of capillary electrophoresis (CE) and micellar electrokinetic capillary chromatography (MEKC) were investigated for the qualitative analysis of some non-steroidal antiinflammatory drugs. In CE the influence of the pH of the buffer and its ionic strength were investigated for a test mixture of six compounds. Also the influence of organic modifiers was studied. The best conditions were applied to the separation of 15 drugs. In MEKC the influence of the concentration of SDS in buffers with pH ranges of 8.0-9.0 was investigated. The influence of an organic modifier, namely acetonitrile was discussed, whereby an interesting phenomenon of change in retention behaviour was noted. A combination of CE and MEKC allows the separation of the 15 above-mentioned compounds and forms an interesting alternative to HPLC.

摘要

研究了毛细管电泳（CE）和胶束电动毛细管色谱法（MEKC）用于某些非甾体抗炎药定性分析的可能性。在毛细管电泳中，研究了缓冲液pH值及其离子强度对六种化合物测试混合物的影响。还研究了有机改性剂的影响。将最佳条件应用于15种药物的分离。在胶束电动毛细管色谱法中，研究了pH范围为8.0 - 9.0的缓冲液中十二烷基硫酸钠（SDS）浓度的影响。讨论了有机改性剂乙腈的影响，由此注意到保留行为变化的有趣现象。毛细管电泳和胶束电动毛细管色谱法的结合能够分离上述15种化合物，形成了一种有趣的高效液相色谱替代方法。