Eberle J, Krasagakis K, Garbe C, Orfanos C E
Universitäts-Hautklinik und Poliklinik, Freien Universität Berlin, Germany.
Melanoma Res. 1993 Apr;3(2):107-12.
In order to enable a direct comparison of gene expression in human melanoma cells and in normal human melanocytes, culture conditions were investigated under which both cell populations can be grown in vitro. Five of 13 melanoma cell lines tested could be cultured to confluence in a melanocyte medium containing fetal calf serum, 12-O-tetradecanoylphorbol-13-acetate and stimulators of cyclic adenosine monophosphate, whereas none could be grown to confluence in a serum-free hormone-supplemented melanocyte medium containing basic fibroblast growth factor and other mitogens. In both melanocyte media the melanoma cells showed signs of increased morphological differentiation with dendrite formation. Normal human melanocytes could be grown in melanoma cell medium for a few days, after which the cultured cells showed a less differentiated phenotype. Since morphological changes may reflect variations in gene expression, we suggest that comparative studies on gene expression of benign and malignant melanocytes should also include thorough investigation under identical culture conditions.
为了能够直接比较人类黑色素瘤细胞和正常人黑色素细胞中的基因表达，研究了两种细胞群体均可在体外生长的培养条件。在含有胎牛血清、12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯和环磷酸腺苷刺激剂的黑色素细胞培养基中，所测试的13种黑色素瘤细胞系中有5种能够培养至汇合，而在含有碱性成纤维细胞生长因子和其他促细胞分裂剂的无血清、添加激素的黑色素细胞培养基中，没有一种能够生长至汇合。在这两种黑色素细胞培养基中，黑色素瘤细胞均表现出形态分化增加并形成树突的迹象。正常人黑色素细胞可在黑色素瘤细胞培养基中生长数天，之后培养的细胞表现出分化程度较低的表型。由于形态变化可能反映基因表达的差异，我们建议对良性和恶性黑色素细胞的基因表达进行比较研究时，还应包括在相同培养条件下的深入研究。
