Cuezva J M, Flores A I, Liras A, Santarén J F, Alconada A
Departamento de Biología Molecular, Universidad Autonoma de Madrid, Spain.
Biol Cell. 1993;77(1):47-62. doi: 10.1016/s0248-4900(05)80174-1.
A review of the proteinaceous machinery involved in protein sorting pathways and protein folding and assembly in mitochondria and peroxisomes is presented. After considering the various sorting pathways and targeting signals of mitochondrial and peroxisomal proteins, we make a comparative dissection of the protein factors involved in: i) the stabilization of cytosolic precursor proteins in a translocation competent conformation; ii) the membrane import apparatus of mitochondria and peroxisomes; iii) the processing of mitochondrial precursor proteins, and the eventual processing of certain peroxisomal precursor, in the interior of the organelles; and iv) the requirement of molecular chaperones for appropriate folding and assembly of imported proteins in the matrix of both organelles. Those aspects of mitochondrial biogenesis that have developed rapidly during the last few years, such as the requirement of molecular chaperones, are stressed in order to stimulate further parallel investigations aimed to understand the origin, biochemistry, molecular biology and pathology of peroxisomes. In this regard, a brief review of findings from our group and others is presented in which the role of the F1-ATPase alpha-subunit is pointed out as a molecular chaperone of mitochondria and chloroplasts. In addition, data are presented that could question our previous indication that the immunoreactive protein found in the rat liver peroxisomes is due to the presence of the F1-ATPase alpha-subunit.
本文综述了参与线粒体和过氧化物酶体中蛋白质分选途径以及蛋白质折叠与组装的蛋白质机器。在考虑了线粒体和过氧化物酶体蛋白质的各种分选途径和靶向信号后，我们对参与以下方面的蛋白质因子进行了比较剖析：i）将胞质前体蛋白稳定在易位活性构象中；ii）线粒体和过氧化物酶体的膜导入装置；iii）线粒体前体蛋白的加工，以及过氧化物酶体某些前体蛋白在细胞器内部的最终加工；iv）分子伴侣对两种细胞器基质中导入蛋白进行适当折叠和组装的需求。强调了线粒体生物发生在过去几年中迅速发展的那些方面，如对分子伴侣的需求，以刺激旨在了解过氧化物酶体的起源、生物化学、分子生物学和病理学的进一步平行研究。在这方面，本文简要综述了我们小组和其他研究小组的发现，其中指出F1 - ATP酶α亚基作为线粒体和叶绿体的分子伴侣的作用。此外，还给出了一些数据，这些数据可能会质疑我们之前关于在大鼠肝脏过氧化物酶体中发现的免疫反应性蛋白是由于F1 - ATP酶α亚基存在的说法。
