Freedman L J, Yi H
Neuroscience Program, University of Iowa, Iowa City 52242.
Brain Res Bull. 1993;31(6):749-51. doi: 10.1016/0361-9230(93)90152-2.
Until now, Fluoro-gold confocal imaging has been problematic because it requires an ultraviolet laser. We describe here a practical method to visualize Fluoro-gold in a confocal laser scanning microscope without an ultraviolet laser through an immunofluorescence procedure using antibodies against Fluoro-gold.
到目前为止,荧光金共聚焦成像一直存在问题,因为它需要紫外激光。我们在此描述一种实用方法,可在没有紫外激光的共聚焦激光扫描显微镜中,通过使用抗荧光金抗体的免疫荧光程序来观察荧光金。