环境温度对妊娠大鼠心房心肌细胞心钠素（ANP）颗粒及血浆ANP水平的影响

Effects of environmental temperature on atrial natriuretic peptide (ANP)-granules of auricular cardiocytes and plasma ANP level in pregnant rats.

作者信息

Mifune H, Suzuki S, Nokihara K, Kobayashi Y, Ueda T, Obara T, Noda Y, Sakamoto H

机构信息

Institute of Animal Experiment, Kurume University School of Medicine, Fukuoka, Japan.

出版信息

Jikken Dobutsu. 1993 Apr;42(2):243-7. doi: 10.1538/expanim1978.42.2_243.

DOI:10.1538/expanim1978.42.2_243

PMID:8519303

Abstract

The atrial natriuretic peptide (ANP) levels of auricular cardiocytes and plasma were examined by immunohistochemistry, ultrastructural morphometry, and radioimmunoassay (RIA) in pregnant rats (4th, 12th, 20th day of gestation) under 22 degrees C and 33 degrees C environmental conditions. Immunohistochemically, ANP immunoreactivity was stronger on the 20th day of gestation in the 22 degrees C environmental groups, but weaker on the 12th and 20th days of gestation in the 33 degrees C environmental groups. According to the results of ultrastructural morphometry, the number of ANP-granules had increased significantly by the 20th day of gestation in the 22 degrees C environmental groups, but was decreased on the 12th and 20th days of gestation in the 33 degrees C environmental groups. RIA demonstrated that the maternal plasma ANP concentration decreased gradually during pregnancy in the 22 degrees C environment. The plasma ANP concentration in the 33 degrees C environmental groups tended to be lower than that at the same stage of pregnancy in the 22 degrees C environmental groups.

摘要

在22摄氏度和33摄氏度环境条件下，采用免疫组织化学、超微结构形态计量学和放射免疫分析法（RIA）检测妊娠大鼠（妊娠第4天、第12天、第20天）心房心肌细胞和血浆中的心房利钠肽（ANP）水平。免疫组织化学显示，在22摄氏度环境组中，妊娠第20天时ANP免疫反应性较强，但在33摄氏度环境组中，妊娠第12天和第20天时ANP免疫反应性较弱。根据超微结构形态计量学结果，在22摄氏度环境组中，妊娠第20天时ANP颗粒数量显著增加，但在33摄氏度环境组中，妊娠第12天和第20天时ANP颗粒数量减少。放射免疫分析表明，在22摄氏度环境中，母体血浆ANP浓度在妊娠期间逐渐降低。33摄氏度环境组的血浆ANP浓度往往低于22摄氏度环境组妊娠相同阶段的浓度。