Kai K, Akagi Y, Soma T, Nomura K, Kanoe M
Department of Veterinary Microbiology, Yamaguchi University, Japan.
J Vet Med Sci. 1995 Aug;57(4):781-3. doi: 10.1292/jvms.57.781.
Kittens inoculated orally with 10(2) PFU of feline enteric coronavirus developed no antibody to the virus despite the repeated challenges. However, they developed antibody for a long period with 5 x 10(3)-1.6 x 10(5) (mean 3 x 10(4)) and with 2.5 x 10(3)-2 x 10(4) (mean 6 x 10(3)) immunoperoxidase antibody titer when they were challenged with 10(5) and 10(3) PFU of virus following previous challenges, respectively. Viremia was found when kittens were inoculated with 10(5) PFU of virus, but not with 10(3) PFU of virus. The dose of 10(3) PFU of virus seemed to be a lower limit to establish infection. These results indicate that local infection induces a low antibody response and systemic infection induces a high antibody response.
口服接种10²个空斑形成单位（PFU）猫肠道冠状病毒的小猫，尽管反复受到攻击，但并未产生针对该病毒的抗体。然而，在先前受到攻击后，分别用10⁵和10³个PFU的病毒对它们进行攻击时，它们产生了长时间的抗体，免疫过氧化物酶抗体滴度分别为5×10³ - 1.6×10⁵（平均3×10⁴）和2.5×10³ - 2×10⁴（平均6×10³）。当小猫接种10⁵个PFU的病毒时发现有病毒血症，但接种10³个PFU的病毒时未发现。10³个PFU的病毒剂量似乎是建立感染的下限。这些结果表明，局部感染诱导低抗体反应，而全身感染诱导高抗体反应。
