Ryu B H, Mao F W, Lou P, Gutman R L, Greenspan P
University of Georgia, College of Pharmacy, Department of Pharmacology and Toxicology, Athens 30602, USA.
Biosci Biotechnol Biochem. 1995 Sep;59(9):1619-22. doi: 10.1271/bbb.59.1619.
The ability of CuSO4- and hypochlorite-oxidized LDL to promote cholesterol accumulation in macrophages was examined. Both CuSO4- and hypochlorite-oxidized LDL were rapidly metabolized by mouse peritoneal macrophages to a level approximately 10 times that observed for native LDL and both modified lipoproteins increased the accumulation of unesterified cholesterol. However when each modified lipoprotein was incubated with macrophages for 40h, only hypochlorite-oxidized LDL produced significant accumulation of cholesteryl esters, with levels approaching 85 micrograms/mg cell protein. This finding was verified by nile red staining. The cholesteryl ester content of cupric sulfate-modified LDL was found to be significantly decreased when compared to either native or hypochlorite-modified LDL promotes massive cholesteryl ester accumulation because the cholesteryl ester content of the LDL particle is preserved.
研究了硫酸铜和次氯酸盐氧化的低密度脂蛋白（LDL）促进巨噬细胞中胆固醇积累的能力。硫酸铜和次氯酸盐氧化的LDL均被小鼠腹腔巨噬细胞迅速代谢至约为天然LDL观察水平的10倍，并且两种修饰的脂蛋白均增加了未酯化胆固醇的积累。然而，当每种修饰的脂蛋白与巨噬细胞孵育40小时时，只有次氯酸盐氧化的LDL产生了显著的胆固醇酯积累，水平接近85微克/毫克细胞蛋白。这一发现通过尼罗红染色得到验证。与天然或次氯酸盐修饰的LDL相比，发现硫酸铜修饰的LDL的胆固醇酯含量显著降低，因为LDL颗粒的胆固醇酯含量得以保留，所以次氯酸盐修饰的LDL促进了大量胆固醇酯的积累。
