Maruyama K, Takatsu A, Obata T
Department of Legal Medicine, Jikei University School of Medicine, Tokyo, Japan.
Biomed Chromatogr. 1995 Jul-Aug;9(4):179-82. doi: 10.1002/bmc.1130090406.
The quantitative analysis of volatile anaesthetics for biomedical applications by means of gas chromatograph/mass spectrometry/selected ion monitoring (GC/MS/SIM) was studied. Xenon gas was selected as an internal standard for the assay by adding to a closed system, because of its stability and inactivity. In the assay of inhalational anaesthetics, isoflurane and nitrous oxide (laughing gas), in forensic samples (serum and cerebrospinal fluid), the calibration of the anaesthetic was linear from 0.12 to 12 nmol/mL in isoflurane and from 30 to 300 nmol/mL in nitrous oxide. Our results suggest that this new method is suitable for the quantitative analysis of inhalational anaesthetics in the biomedical field.
研究了采用气相色谱/质谱/选择离子监测（GC/MS/SIM）对生物医学应用中的挥发性麻醉剂进行定量分析。由于氙气的稳定性和惰性，通过添加到封闭系统中，将其选作该测定的内标。在法医样本（血清和脑脊液）中对吸入性麻醉剂异氟烷和一氧化二氮（笑气）进行测定时，异氟烷麻醉剂的校准在0.12至12 nmol/mL范围内呈线性，一氧化二氮在30至300 nmol/mL范围内呈线性。我们的结果表明，这种新方法适用于生物医学领域中吸入性麻醉剂的定量分析。
