Lind H, Erlinge D, Brunkwall J, Edvinsson L
Department of Internal Medicine, Lund University, Sweden.
Clin Auton Res. 1995 Sep;5(4):191-7. doi: 10.1007/BF01824006.
Vascular smooth muscle contractile responses to neuropeptide Y, alpha,beta-methyleneATP and noradrenaline were studied in circular segments of isolated vessels with intact endothelium in vitro from 12 patients with diabetes mellitus type 2 (NIDDM) and 12 control subjects. The dilatory effect of acetylcholine was used to test the function of the endothelium. Subcutaneous arteries and veins (diameter 0.1-1.1 mm) were obtained during surgery. There was no difference in contractile responses to noradrenaline or alpha,beta-methyleneATP between diabetic and control vessels. The contractile response to neuropeptide Y, however, was markedly reduced in the diabetic group. The maximal contractile effect (46.0 +/- 14.0%, p < 0.05) but not the sensitivity to neuropeptide Y was significantly less in diabetic veins compared to control (107.5 +/- 19.6%). Thus, the attenuation of neuropeptide Y responses was present in humans as previously observed in alloxan-induced diabetes mellitus in rabbits. There was no difference in the dilator effect of acetylcholine between the diabetic and the control group in any of the vessel types, indicating that the difference in vascular reactivity to neuropeptide Y was not endothelium-dependent. In conclusion, the present study has shown that the postjunctional effects of neuropeptide Y, a co-transmitter of the peripheral sympathetic nervous system, is selectively attenuated in diabetes mellitus.
在体外对12名2型糖尿病（非胰岛素依赖型糖尿病，NIDDM）患者和12名对照受试者分离的具有完整内皮的血管环段，研究了血管平滑肌对神经肽Y、α,β-亚甲基ATP和去甲肾上腺素的收缩反应。用乙酰胆碱的舒张作用来测试内皮功能。手术中获取皮下动脉和静脉（直径0.1 - 1.1毫米）。糖尿病血管和对照血管对去甲肾上腺素或α,β-亚甲基ATP的收缩反应没有差异。然而，糖尿病组对神经肽Y的收缩反应明显降低。与对照组（107.5±19.6%）相比，糖尿病静脉对神经肽Y的最大收缩效应（46.0±14.0%，p<0.05）显著降低，但对神经肽Y的敏感性没有差异。因此，正如之前在兔的四氧嘧啶诱导糖尿病中所观察到的，人类也存在神经肽Y反应的减弱。在任何血管类型中，糖尿病组和对照组之间乙酰胆碱的舒张作用没有差异，这表明血管对神经肽Y反应性的差异不是内皮依赖性的。总之，本研究表明，作为外周交感神经系统的一种共同递质，神经肽Y的节后效应在糖尿病中选择性减弱。
