Apffel A, Chakel J, Udiavar S, Hancock W S, Souders C, Pungor E
Hewlett-Packard Laboratories, Palo Alto, CA 94304, USA.
J Chromatogr A. 1995 Nov 24;717(1-2):41-60. doi: 10.1016/0021-9673(95)00603-0.
The analysis of recombinant Desmodus salivary plasminogen activator (DSPA alpha 1), a heterogeneous glycoprotein, is demonstrated through the use of high-performance liquid chromatography (HPLC), high-performance capillary electrophoresis (HPCE), liquid chromatography-electrospray mass spectrometry (LC--ES-MS), and matrix-assisted laser desorption ionization--time of flight mass spectrometry (MALDI--TOF-MS). The proteins is analyzed at three specific levels of detail: the intact protein, proteolytic digests of the protein, and fractions from the proteolytic digest. A method for "on-column" collection of HPLC fractions for subsequent transfer and analysis by HPCE and MALDI--TOF-MS is shown.
通过使用高效液相色谱（HPLC）、高效毛细管电泳（HPCE）、液相色谱 - 电喷雾质谱（LC - ES - MS）和基质辅助激光解吸电离 - 飞行时间质谱（MALDI - TOF - MS），对重组吸血蝠唾液纤溶酶原激活剂（DSPAα1）这一异质糖蛋白进行了分析。该蛋白质在三个特定的详细程度水平上进行分析：完整蛋白质、蛋白质的蛋白水解消化物以及蛋白水解消化物的馏分。展示了一种用于“柱上”收集HPLC馏分以便随后通过HPCE和MALDI - TOF - MS进行转移和分析的方法。
