Lack G, Oshiba A, Bradley K L, Loader J E, Amran D, Larsen G L, Gelfand E W
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA.
Am J Respir Crit Care Med. 1995 Dec;152(6 Pt 1):1765-73. doi: 10.1164/ajrccm.152.6.8520735.
The role of allergen-specific sIgE+ B cells in the development of airway hyperresponsiveness to electrical field stimulation was examined in a murine model of allergic sensitization. Ovalbumin (OVA)-specific B cells (OVA+) were isolated from mice that were sensitized to aerosolized OVA. The OVA+ B cell population was shown to be distinct from the remaining, non-OVA-responsive B cells (OVA-). There was a high frequency of sIgE+ B cells and a low frequency of sIgG+ B cells in the OVA+ population compared with the OVA- population, where the ratio was reversed. Although both populations produced immunoglobulin in vitro, only the OVA+ cells secreted anti-OVA antibodies. Transfer of 10(6) OVA+ B cells or as few as 5 x 10(4) OVA+/sIgE+ B cells was able to transfer the capability for anti-OVA IgE synthesis and cutaneous reactivity to OVA in naive recipients. Exposure to OVA via the airways in addition to transfer of OVA+ B cells was necessary for development of airway hyperresponsiveness, whereas recipients challenged with an irrelevant allergen, ragweed, had normal airway function. Transfer of up to 10(7) OVA- B cells failed to induce production of anti-OVA IgE. Despite production of polyclonal IgE, recipients of OVA- B cells did not develop airway hyperresponsiveness after OVA challenge. We conclude that both allergen-specific IgE production and local challenge via the airways with specific allergen are necessary to change airway function in this model.
在过敏性致敏的小鼠模型中，研究了变应原特异性sIgE+B细胞在气道对电场刺激的高反应性发展中的作用。从对雾化卵清蛋白（OVA）致敏的小鼠中分离出OVA特异性B细胞（OVA+）。结果显示，OVA+B细胞群体与其余非OVA反应性B细胞（OVA-）不同。与OVA-群体相比，OVA+群体中sIgE+B细胞频率高，sIgG+B细胞频率低，而在OVA-群体中这一比例则相反。虽然两个群体在体外都产生免疫球蛋白，但只有OVA+细胞分泌抗OVA抗体。将10⁶个OVA+B细胞或低至5×10⁴个OVA+/sIgE+B细胞转移到未致敏的受体中，能够转移抗OVA IgE合成能力和对OVA的皮肤反应性。除了转移OVA+B细胞外，通过气道接触OVA对于气道高反应性的发展是必要的，而用无关变应原豚草攻击的受体气道功能正常。转移多达10⁷个OVA-B细胞未能诱导抗OVA IgE的产生。尽管产生了多克隆IgE，但OVA-B细胞受体在OVA攻击后并未出现气道高反应性。我们得出结论，在该模型中，变应原特异性IgE的产生以及通过气道用特异性变应原进行局部攻击对于改变气道功能都是必要的。
