Kovács P, Baricová L, Kovalová M, Dostál J, Stankovicová T, Svec P
Katedra farmakológie a toxikológie Farmaceutickej fakulty Univerzity Komenského, Bratislava.
Ceska Slov Farm. 1995 Oct;44(5):257-60.
The present study aims to investigate the effects of the lipophilic antioxidant Trolox C (a vitamin E analogue) and stobadine, a scavenger of free oxygen radicals, on reperfusion dysrhythmias. Experiments were performed on isolated perfused rat hearts subjected to global stop-flow ischaemia followed by reperfusion. Trolox C (10(-4 mol.l-1) and stobadine (10(-5) mol.l-1) were infused immediately prior to ischaemia. Trolox C (10(-4) mol.l-1) and stobadine (10(-5) mol.l-1) decreased the incidence and duration of reperfusion-induced dysrhythmias (quantified by the dysrhythmia score) in comparison to the ischaemic-reperfusion damaged hearts. There was an improvement in the recovery of contraction force and left ventricular diastolic pressure in Trolox or stobadine pretreated hearts. No significant changes in coronary flow resistance were observed. The results suggest that both substances protect the myocardium during ischaemic-reperfusion injury probably by affecting the generation and activity of reactive oxygen species.
本研究旨在探讨亲脂性抗氧化剂生育酚（维生素E类似物）和氧自由基清除剂司巴丁对再灌注性心律失常的影响。实验采用离体灌注大鼠心脏，先进行全心停流缺血，然后再灌注。在缺血前立即注入生育酚（10⁻⁴mol·L⁻¹）和司巴丁（10⁻⁵mol·L⁻¹）。与缺血-再灌注损伤心脏相比，生育酚（10⁻⁴mol·L⁻¹）和司巴丁（10⁻⁵mol·L⁻¹）可降低再灌注诱导的心律失常的发生率和持续时间（通过心律失常评分量化）。生育酚或司巴丁预处理的心脏收缩力和左心室舒张压的恢复有所改善。未观察到冠状动脉血流阻力有显著变化。结果表明，这两种物质可能通过影响活性氧的产生和活性，在缺血-再灌注损伤期间保护心肌。
