Ermolaeva O D, Wagner M C
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
Comput Appl Biosci. 1995 Aug;11(4):457-62. doi: 10.1093/bioinformatics/11.4.457.
We report on a new software tool, SUBTRACT, which allows the analysis of the possible strategies for an experiment based on a mathematical model of subtractive hybridization. The program helps the experimenter choose the optimal strategy and conditions for performing the reaction. The program allows the modeling of cDNA and genomic subtraction for different types of target DNA. SUBTRACT offers a friendly interface for the investigation of the dynamics of the process of subtractive hybridization, and for modifying different parameters and initial conditions. SUBTRACT allows the user to plot the values of interesting quantities as a function of the reaction time. The program runs under the Unix operating system on Sun-compatible computers.
我们报告了一种新的软件工具SUBTRACT，它能够基于消减杂交的数学模型分析实验的可能策略。该程序帮助实验者选择进行反应的最佳策略和条件。该程序允许对不同类型的靶DNA进行cDNA和基因组消减的建模。SUBTRACT为研究消减杂交过程的动力学以及修改不同参数和初始条件提供了友好的界面。SUBTRACT允许用户绘制感兴趣的量的值作为反应时间的函数。该程序在与Sun兼容的计算机上的Unix操作系统下运行。
